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Foxp3/Transcription Factor Flow Cytometry Fixation And Permeabilization Breaking Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Flow Cytometry Fixation Buffer Cat. Pf00016, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flow cytometry fixation buffer cat. pf00016/product/Proteintech
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Permeabilization Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Fixation Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
1x Fixation Permeabilization Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Permeabilization Buffer Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/permeabilization buffer kit/product/R&D Systems
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

Journal: Journal of Inflammation Research

Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway

doi: 10.2147/JIR.S509734

Figure Lengend Snippet: The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

Article Snippet: Flow cytometry fixation buffer (Cat. PF00016), and Flow Cytometry Perm Buffer (Cat. PF00017) were purchased from Proteintech (Hubei, China).

Techniques: Flow Cytometry, Expressing, Co-Immunoprecipitation Assay